Maliha Zahid, M.D., Ph.D.

  • Assistant Professor

Research Interests

My current research interests can be broadly classified into two categories. My first interest is of a translational nature where I am interested in identifying genes mutations in which would be responsible for disrupting normal cardiac development and leading to various forms of congenital heart disease. The heart is a highly asymmetric organ, necessary for producing a blood circuit in parallel leading to deoxygenated blood being delivered to the lungs and oxygenated blood to the rest of the body. The primary cilia, at the embryonic node, is key for breaking the initial symmetry of the developing embryo. We hypothesize that mutations in cilia related gene affecting its function both as a motile as well as a key organelle for sensory and transcriptional activity will be reflected both in laterality defects affecting the heart and also as abnormal ciliary motion in the respiratory epithelia. To study this, we have recruited over 400 patients and family members with various simple and complex forms of congenital heart disease and studied their respiratory ciliary function as well as carry out whole genome sequencing to identify mutations in known cilia related genes.

The second broad category of studies I am carrying out relate to mechanisms of normal ciliogenesis. We have been able to grow human and mouse respiratory epithelia in the lab and get them to reciliated. This process not only allows us to confirm nasal ciliary motion calls (in human nasal epithelial scrapes) but also provides us with the opportunity to study the process of ciliogenesis in vitro. I am interested in the normal process leading to ciliogenesis as well as changes in the process with age. Furthermore, I am looking at hedgehog signaling, an important and early transcription factor in embryonic development, as it relates to normal cilia development. Hedgehog signaling cascade involves certain key players in the pathway moving in and out of the primary cilia. However the role, if any, of hedgehog signaling in ciliogenesis remains unexplored.

Representative Publications

Zahid M, Kapila A, Eagan CE, Yusko DA, Miller ED, Missenda CD. Prevalence and significance of electrocardiographic changes and side effect profile of regadenoson compared with adenosine during myocardial perfusion imaging. 2013; 4(1): 7-10.
[Abstract]

Rockacy MJ, Zahid M, McGrath KM, Fasanella KE, Khalid A. Association between RAS Mutation, Detected in Pancreatic Cyst Fluid, and Long-Term Outcomes of Patients. Clin Gastroenterol Hepatol. 2013 Apr;11(4):425-9. 
[Abstract]

Zahid M, Singla I, Good CB, Stone RA, Kim S, Fine MJ, Sonel AF. Associations between Pneumococcal Vaccination and Adverse Outcomes in Patients with Suspected Acute Coronary Syndrome. Advances in Infectious Diseases. 2012 December: 122-134. 
[Abstract]

Zahid M, Robbins PD. Protein Transduction Domains: Applications for Molecular Medicine. Curr Gene Ther. 2012;12(5):374-80.
[Abstract]

Zahid M, Arora S. Reverse Wenckebach "pseudo-supernormal" conduction or paroxysmal atrioventricular block. J Cardiovasc Dis Res. 2012 Jul;3(3):225-7.
[Abstract]

Kotchey NM, Adachi K, Zahid M, Inagaki K, Charan R, Parker RS, Nakai H. A potential role of distinctively delayed blood clearance of recombinant adeno-associated virus serotype 9 in robust cardiac transduction. Mol Ther. 2011;19(6):1079-89. 
[Abstract]

Zahid M, Robbins PD. Identification and characterization of tissue-specific protein transduction domains using Peptide phage display. Methods Mol Biol. 2011;683:277-89.
[Abstract]

Zahid M, Lu X, Mi Z, Robbins PD. Cationic and Tissue-Specific Protein Transduction Domains Identification, Characterization, and Therapeutic Application. Adv Genet. 2010;69C:83-95.
[Abstract]

Zahid M, Good CB, Sonel AF (2010). Differential Diagnosis of Serum Cardiac Troponin Elevation: A Gold Standard for Diagnosing Acute Myocardial Injury. Chambers & Valves, the Journal of Cardiology. ]

Zahid M, Phillips BE, Albers SM, Giannoukakis N, Watkins SC, Robbins PD (2010). Identification of a Cardiac Specific Protein Transduction Domain by In Vivo Biopanning Using a M13 Phage Peptide Display Library in Mice. PLoS ONE 5(8): e12252. doi:10.1371/journal.pone.
[Abstract]